Biosafety Cabinet
There are many types of Biosafety Cabinet. Class I, Class IIA, Class IIB1, Class IIB2, Class IIB3 and Class III.
Class I BSC allow air to pass into the cabinet without passing it through HEPA filter and this tell us that it does not provide protection against the product. The exhaust is equipped with a HEPA filter so that the air will be free from contaminant before it is allowed to exit into the environment. Thus, it has a good protection to the laboratory personnel and the environment. The air flow rate of Class I BSC is about 75fpm. It is often used to enclosed equipment such as fermenter or any process that produce a lot of aerosol.
Class IIA biosafety cabinet has a front and back grille. The air is drawn into the HEPA filter from the front grille and 70% of the filtered air is introduced from the top of the BSC and the remaining 30% is goes to the exhaust. This provide protection to the product, personel and the environment. The air flow rate is 75fpm.
Class IIB1 biosafety cabinet has an air flow of 100fpm and there are front and back grilles. About 70% of the air is drawn from the back grille and 30% from the front grille so when you want to work with chemical that is cacinogens, it is recommended for you to do at the rear grille due to the high air change at that particular area. This system has a 30% air circulated into the cabinet while 70% exit to the exhaust.
Class IIB2 biosafety cabinet has a 100% air exhausted. The air will first enter the HEPA filter before it is introduced into the cabinet so it protect the products. The air is also filtered after it has been passed the cabinet so that it protects the environment. The front grille suck the air inward so it protects against the laborartory personnel.
Class IIB3 is the same as Class IIB2 but it has a negative pressure that protect the cabinet so that if there is any leaks, there will be no chance for it to enter the environment. The negative pressure front grille make sure that the air enter the BSC but not exit into the environment. It has a positive pressure contaminated plenums within the cabinet are surrounded by a negative air pressure planum so leakage in a contaminated plenumwill be into the cabinet and not into the environment.
Class III is the glove box and it is a closed system to be used in BSL-4 virus. It has a very good protection and have 100fpm air velocity. The outlet is equipped with 2 layers of HEPA filter for better air filtration. It is a gas tight contruction with non opening view window. It provides a maximum protection to personnel and the environment.
Class I BSC allow air to pass into the cabinet without passing it through HEPA filter and this tell us that it does not provide protection against the product. The exhaust is equipped with a HEPA filter so that the air will be free from contaminant before it is allowed to exit into the environment. Thus, it has a good protection to the laboratory personnel and the environment. The air flow rate of Class I BSC is about 75fpm. It is often used to enclosed equipment such as fermenter or any process that produce a lot of aerosol.
Class IIA biosafety cabinet has a front and back grille. The air is drawn into the HEPA filter from the front grille and 70% of the filtered air is introduced from the top of the BSC and the remaining 30% is goes to the exhaust. This provide protection to the product, personel and the environment. The air flow rate is 75fpm.
Class IIB1 biosafety cabinet has an air flow of 100fpm and there are front and back grilles. About 70% of the air is drawn from the back grille and 30% from the front grille so when you want to work with chemical that is cacinogens, it is recommended for you to do at the rear grille due to the high air change at that particular area. This system has a 30% air circulated into the cabinet while 70% exit to the exhaust.
Class IIB2 biosafety cabinet has a 100% air exhausted. The air will first enter the HEPA filter before it is introduced into the cabinet so it protect the products. The air is also filtered after it has been passed the cabinet so that it protects the environment. The front grille suck the air inward so it protects against the laborartory personnel.
Class IIB3 is the same as Class IIB2 but it has a negative pressure that protect the cabinet so that if there is any leaks, there will be no chance for it to enter the environment. The negative pressure front grille make sure that the air enter the BSC but not exit into the environment. It has a positive pressure contaminated plenums within the cabinet are surrounded by a negative air pressure planum so leakage in a contaminated plenumwill be into the cabinet and not into the environment.
Class III is the glove box and it is a closed system to be used in BSL-4 virus. It has a very good protection and have 100fpm air velocity. The outlet is equipped with 2 layers of HEPA filter for better air filtration. It is a gas tight contruction with non opening view window. It provides a maximum protection to personnel and the environment.
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